mcherry tag  (Genecopoeia)

Journal: Cell & Bioscience

Article Title: The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries

doi: 10.1186/s13578-026-01530-1

Figure Lengend Snippet: Three types of dynamic changes in cytoophidia. A Live-cell imaging of cytoophidium (arrow) transporting from nurse cell (nc) to oocyte (oo). CTPS is tagged with mCherry (red), ring canal is labeled by GFP-Pav (green). Time stamps are on the top-left, images are taken for every 4 min. Scale bar, 20 μm (left), 5 μm (right). B Live-cell imaging of cytoophidium (arrow) transporting from one nurse cell to the other nurse cell. CTPS is tagged with mCherry (red), actin labeled by CellMask Green Actin Tracking Stain (green). Time stamps are on the top-left, images are taken for every 4 min. Scale bar, 5 μm. C Trajectories of cytoophidia in germline cells. CTPS is tagged with mCherry (red). Time stamps are on the top-left, images are taken for every 4 min. Time stamps are on the top-left, images are taken for every 4 min. Scale bar, 5 μm. D Trajectories of cytoophidia in follicle cells. CTPS is tagged with mCherry (gray). Time stamps are on the top-left, images are taken for every 4 min. Time stamps are on the top-left, images are taken for every 4 min. Scale bar, 5 μm.

Article Snippet: It was then incubated with the following primary antibodies: anti-mCherry tag monoclonal antibody (Abbkine, A02080) to label CTPS, and anti-GAPDH monoclonal antibody (Novus, NB300-221SS) as the internal control.

Techniques: Live Cell Imaging, Labeling, Staining

Journal: Cell & Bioscience

Article Title: The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries

doi: 10.1186/s13578-026-01530-1

Figure Lengend Snippet: Microtubules are essential for cytoophidium morphological changes, movement and assembly. A Time-lapse imaging revealed cytoophidia morphological changes, The time marked in the upper left corner represents the time since the capture began. The CTPS (gray) signal shown is obtained using mCherry-tagged CTPS. Scale bar = 5 μm. B Cytoophidia abundance and length are affected by microtubule depolymerization. Microtubules (green) are labeled with FITC-conjugated tubulin antibody. CTPS (red) signal is obtained using mCherry-tagged CTPS. scale bars, 20 μm. C Quantification of cytoophidium intercellular transportation through nurse cell-oocyte ring canals in control and microtubule inhibited groups. We analyzed 25 ovaries in control group and 25 ovaries in microtubule depolymerization group. D The speed of cytoophidia movement. We analyzed 35 filaments in control group and 35 filaments in microtubule depolymerization group. Data are represented as mean ± SEM. *** p < 0.001. E Length of cytoophidia in ovary nurse cell. We analyzed 32 filaments in control group and 88 filaments in microtubule depolymerization. Data are represented as mean ± SEM. *** p < 0.001. F Number of cytoophidia in ovary nurse cell. We analyzed 9 ovaries in control group and 413 filaments in total, and 9 ovaries in microtubule depolymerization and 1417 filaments in total, Data are represented as mean ± SEM. ** p < 0.001. G Western blotting analysis of CTPS proteins from ovary of CTPS-mCherry fly. Anti-mCherry antibody was used for the immunoblotting analysis. GAPDH was used as an internal control. H The CTPS protein levels in the samples from G were quantified. Data are represented as mean ± SEM. ns, no significance in difference. I Measurement of the relative CTPS mRNA expression was conducted via quantitative RT-PCR using ovary from the control and MT inhibitor-treated flies (6 ovaries/group, 3 biological replicates). Data are represented as mean ± SEM. ns, no significance in difference

Article Snippet: It was then incubated with the following primary antibodies: anti-mCherry tag monoclonal antibody (Abbkine, A02080) to label CTPS, and anti-GAPDH monoclonal antibody (Novus, NB300-221SS) as the internal control.

Techniques: Imaging, Labeling, Control, Western Blot, Expressing, Quantitative RT-PCR

Journal: Cell & Bioscience

Article Title: The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries

doi: 10.1186/s13578-026-01530-1

Figure Lengend Snippet: Microtubules stabilization does not affect cytoophidium morphological changes, movement and assembly. A Time-lapse imaging revealed cytoophidia morphological changes, The time marked in the upper left corner represents the time since the capture began. The CTPS (gray) signal shown is obtained using mCherry-tagged CTPS. Scale bar = 5 μm. B Cytoophidia abundance and length are not affected by microtubule polymerization. Microtubules (green) are labeled with FITC-conjugated tubulin antibody. CTPS (red) signal is obtained using mCherry-tagged CTPS. scale bars, 20 μm. C Quantification of cytoophidia transport through nurse cell-oocyte ring canals in control and microtubules polymerization groups. We analyzed 14 ovaries in control group and 14 ovaries in microtubule polymerization group. D The speed of cytoophidia movement. We analyzed 47 filaments in control group and 47 filaments in microtubule polymerization group. Data are represented as mean ± SEM. ns, no significance in difference. E Number of cytoophidia in ovary nurse cell. We analyzed 5 ovaries in control group and 245 filaments in total, and 5 ovaries in microtubule polymerization and 233 filaments in total. Data are represented as mean ± SEM. ns, no significance in difference. F Length of cytoophidia in ovary nurse cell. We analyzed 47 filaments in control group and 47 filaments in microtubule polymerization. Data are represented as mean ± SEM. ns, no significance in difference. G Intensity of CTPS-mCherry fluorescence signal in cytoophidia (ns, no significance in difference). 3 ovaries in control group and 3 ovaries in MT stabilizer-treated group are analyzed. Data are represented as mean ± SEM.

Article Snippet: It was then incubated with the following primary antibodies: anti-mCherry tag monoclonal antibody (Abbkine, A02080) to label CTPS, and anti-GAPDH monoclonal antibody (Novus, NB300-221SS) as the internal control.

Techniques: Imaging, Labeling, Control, Fluorescence

Journal: Cell & Bioscience

Article Title: The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries

doi: 10.1186/s13578-026-01530-1

Figure Lengend Snippet: Dynein is indispensable for assembly and directed movement of cytoophidia. A Time-lapse imaging revealed cytoophidia morphological changes, The time marked in the upper left corner represents the time since the capture began. The CTPS (gray) signal shown is obtained using mCherry-tagged CTPS. Scale bar = 5 μm. B Quantification of cytoophidia transport through nurse cell-oocyte ring canals in control and dynein inhibited groups. We analyzed 16 ovaries in control group and 18 ovaries in dynein inhibited group. C The speed of cytoophidia movement. We analyzed 35 filaments in control group and 35 filaments in dynein inhibited group. Data are represented as mean ± SEM. ns, no significance in difference. D Length of cytoophidia in ovary nurse cell. We analyzed 43 filaments in control group and 47 filaments in dynein inhibited. Data are represented as mean ± SEM. *** p < 0.001. E Number of cytoophidia in ovary nurse cell. We analyzed 8 ovaries in control group and 265 filaments in total, and 8 ovaries in dynein inhibited and 557 filaments in total. Data are represented as mean ± SD. **** p < 0.0001. F Quantitative RT-PCR was used to assess the relative expression levels of CTPS mRNA. Ovaries were collected from the control and dynein inhibitor-treated flies (6 ovaries/group, 3 biological replicates). Data are represented as mean ± SEM. ns, no significance in difference. G Intensity of CTPS-mCherry signal in cytoophidia (ns, no significance in difference). 3 ovaries in control group and 3 ovaries in dynein inhibited group are analyzed. Data are represented as mean ± SEM.

Article Snippet: It was then incubated with the following primary antibodies: anti-mCherry tag monoclonal antibody (Abbkine, A02080) to label CTPS, and anti-GAPDH monoclonal antibody (Novus, NB300-221SS) as the internal control.

Techniques: Imaging, Control, Quantitative RT-PCR, Expressing

Journal: Cell & Bioscience

Article Title: The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries

doi: 10.1186/s13578-026-01530-1

Figure Lengend Snippet: Kinesin does not affect cytoophidia dynamic changes. A Time-lapse imaging revealed cytoophidia morphological changes, The time marked in the upper left corner represents the time since the capture began. The CTPS (gray) signal shown is obtained using mCherry-tagged CTPS. Scale bar = 5 μm. B Khc knockdown Drosophila ovaries. CTPS (red) signal shown is obtained using mCherry-tagged CTPS. Nuclei(blue) are stained by Hoechst 33342. scale bars, 20 μm. C Quantification of cytoophidia transport through nurse cell-oocyte ring canals in control and Khc knockdown groups. We analyzed 30 ovaries in control group and 25 ovaries in Khc knockdown group. D The speed of cytoophidia movement. We analyzed 41 filaments in control group and 30 filaments in Khc knockdown group, Data are represented as mean ± SEM. ns, no significance in difference. E Length of cytoophidia in ovary nurse cell. We analyzed 43 filaments in control group and 44 filaments in Khc knockdown, Data are represented as mean ± SEM. ns, no significance in difference. F Number of cytoophidia in nurse cell. We analyzed 6 ovaries in control group and 162 filaments in total, and 6 ovaries in Khc knockdown and 184 filaments in total, Data are represented as mean ± SEM. ns, no significance. G Intensity of CTPS-mCherry fluorescence signal in cytoophidia (ns, no significance in difference). 3 ovaries in control group and 3 ovaries in Khc group are analyzed. Data are represented as mean ± SEM.

Article Snippet: It was then incubated with the following primary antibodies: anti-mCherry tag monoclonal antibody (Abbkine, A02080) to label CTPS, and anti-GAPDH monoclonal antibody (Novus, NB300-221SS) as the internal control.

Techniques: Imaging, Knockdown, Staining, Control, Fluorescence

Journal: Cell & Bioscience

Article Title: The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries

doi: 10.1186/s13578-026-01530-1

Figure Lengend Snippet: Microfilaments are essential for movement, morphological changes and assembly of cytoophidia. A Time-lapse imaging revealed cytoophidia morphological changes, The time marked in the upper left corner represents the time since the capture began. The CTPS (gray)signal shown is obtained using mCherry-tagged CTPS. Scale bar = 5 μm. B Cytoophidia abundance and length are affected by microfilaments depolymerization. Microfilaments (green) are labeled with phalloidin, CTPS (red) signal shown is obtained using mCherry-tagged CTPS. scale bars, 20 μm. C Quantification of cytoophidia transport through nurse cell-oocyte ring canals in control and microfilaments depolymerization groups. We analyzed 27 ovaries in control group and 27 ovaries in microfilaments depolymerization group. D The speed of cytoophidia movement. We analyzed 48 filaments in control group and 48 filaments in microfilaments depolymerization group. Data are represented as mean ± SEM, * p < 0.05. E Length of cytoophidia in ovary nurse cell. We analyzed 184 filaments in control group and 184 filaments in microfilaments depolymerization. Data are represented as mean ± SEM, *** p < 0.001. F Number of cytoophidia in ovary nurse cell. We analyzed 9 ovaries in control group and 450 filaments in total, and 9 ovaries in microfilaments depolymerization and 835 filaments in total. Data are represented as mean ± SEM, ** p < 0.01. G The relative expression of CTPS mRNA was acquired and quantified utilizing quantitative PCR using ovary from the control and microfilaments inhibitor-treated flies (6 ovaries/group, 3 biological replicates). Data are represented as mean ± SEM, ns, no significance. H Intensity of CTPS-mCherry signal in cytoophidia (ns, no significance in difference). 3 ovaries in control group and 3 ovaries in microfilaments inhibited group are analyzed. Data are represented as mean ± SEM.

Article Snippet: It was then incubated with the following primary antibodies: anti-mCherry tag monoclonal antibody (Abbkine, A02080) to label CTPS, and anti-GAPDH monoclonal antibody (Novus, NB300-221SS) as the internal control.

Techniques: Imaging, Labeling, Control, Expressing, Real-time Polymerase Chain Reaction

Journal: Cell & Bioscience

Article Title: The cytoskeleton regulates cytoophidium dynamics in Drosophila ovaries

doi: 10.1186/s13578-026-01530-1

Figure Lengend Snippet: Myosin is essential for movement, morphological changes and assembly of cytoophidia. A Time-lapse imaging revealed cytoophidia morphological changes, The time stamps represent the time since the capturing began. The CTPS (gray) signal shown is obtained using mCherry-tagged CTPS. Scale bar = 5 μm. B Quantification of cytoophidia transport through nurse cell-oocyte ring canals in control and myosin inhibited groups. We analyzed 19 ovaries in control group and 19 ovaries in myosin inhibited group. C The speed of cytoophidia movement. We analyzed 56 filaments in control group and 56 filaments in myosin inhibited group. Data are represented as mean ± SEM, *** p < 0.001. D Number of cytoophidia in ovary nurse cell. We analyzed 10 ovaries in control group and 581 filaments in total, and 10 ovaries in myosin inhibited and 989 filaments in total. Data are represented as mean ± SEM, *** p < 0.001. E Length of cytoophidia in ovary nurse cell. We analyzed 94 filaments in control group and 94 filaments in myosin inhibited. Data are represented as mean ± SEM, ** p < 0.01. F Relative mRNA levels of control and myosin inhibitor-treated are measured by quantitative RT-PCR (6 ovaries/group, 3 biological replicates). Data are represented as mean ± SEM. ns, no significance. G Intensity of CTPS-mCherry signal in cytoophidia (ns, no significance in difference). 3 ovaries in control group and 3 ovaries in myosin inhibited group are analyzed. Data are represented as mean ± SEM.

Article Snippet: It was then incubated with the following primary antibodies: anti-mCherry tag monoclonal antibody (Abbkine, A02080) to label CTPS, and anti-GAPDH monoclonal antibody (Novus, NB300-221SS) as the internal control.

Techniques: Imaging, Control, Quantitative RT-PCR